Secretion of alpha-amylase from parotid lobules of young and old rats

The rate of release of a secretory enzyme, alpha-amylase, from the parotid lobules of 2 and 24 month old rats has been compared to determine whether the secretory activity of the cells change during aging. Upon incubation in the presence of a secretogogue, isoproterenol (10(-5) M), about the same proportions of the glandular alpha-amylase are released at about the same rate from these lobules. The isoproterenol-stimulated release of the enzyme is inhibited nearly completely by preincubating the lobules with propranolol (10(-5) M) in both age groups, indicating that the enzyme secretion occurs through the stimulation of beta-receptors. When viewed in the electron microscope, the cell membranes bordering the secretory lumen in isoproterenol-incubated lobules reveal festooned appearances which suggest that the enzyme release occurs by means of exocytosis. These observations indicate that the ability of the parotid cells to release secretory products through the beta-receptor mediation does not significantly change with increasing age.