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[Rheologic activation of blood platelets. Preliminary study]
Journal Des Maladies Vasculaires
|January 1, 1983
Summary
High shear stress impacts blood cells, activating platelets more rapidly in platelet-rich plasma than in whole blood. This study quantifies shear-induced cell damage and platelet activation.
Area of Science:
- Biomedical Engineering
- Hematology
- Rheology
Context:
- Investigating the mechanical forces on blood components is crucial for understanding hemorheology and potential thrombotic events.
- Blood cells, including platelets and red blood cells, are subjected to varying shear rates in physiological and pathological conditions.
Purpose:
- To determine the effect of high deformation rates (up to 20,000/s) on blood platelets, with and without red blood cells.
- To assess red blood cell lysis and platelet activation under shear stress using biochemical markers.
Summary:
- A Couette viscosimeter subjected whole blood and platelet-rich plasma (PRP) to shear rates up to 20,000/s.
- Red blood cell changes were observed above 10,000/s, correlating with increased lactate dehydrogenase (LDH) and hemoglobin release.
- Platelet activation, indicated by beta-thromboglobulin (beta-TG) release, occurred earlier and more rapidly in PRP compared to whole blood at higher shear rates.
Impact:
- Findings suggest that high shear stress can induce significant mechanical and biochemical changes in blood components.
- Understanding these shear-dependent responses is vital for developing strategies to mitigate blood cell damage in medical devices and cardiovascular diseases.