Distribution of peripherally stained neurons by the colloidal iron histochemical method in albino rat cerebral...

A few stained neurons were demonstrated in several cortical areas by the Colloidal Iron method. The intense positive histochemical reaction was located surrounding the perikaryon and some neuronal branches. A quantitative study demonstrated that the largest percentage of stained neurons was found in III, IV and V layers of the visual and auditory cortex. The number of stained neurons in the auditory cortex was greater than in the visual cortex. Statistically significant differences (Student's t-test) were obtained between layers I, II, III and VI of both cortical areas. In other cortical areas studied there were no stained neurons (area insularis ventralis, area entorhinalis and area piriformis).