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In vitro assessment of platelet damage during rotator storage
American Journal of Clinical Pathology
|June 1, 1983
Summary
Platform and circular rotators cause less platelet damage during storage than elliptical rotators. This is shown by lower levels of beta-thromboglobulin (BTG) and lactate dehydrogenase (LDH) release, indicating reduced platelet activation and lysis.
Area of Science:
- Transfusion Medicine
- Hematology
- Biotechnology
Background:
- Platelet concentrates are vital for treating bleeding disorders.
- Damage during preparation and storage can affect platelet efficacy.
- Beta-thromboglobulin (BTG) and lactate dehydrogenase (LDH) indicate platelet activation and lysis.
Purpose of the Study:
- To compare the impact of different rotator types on platelet concentrate quality.
- To assess BTG and LDH levels as indicators of platelet damage under various agitation methods.
Main Methods:
- Platelet concentrates were stored on platform, elliptical, and circular rotators.
- BTG and LDH concentrations in supernatant plasma were measured over time.
- Statistical analysis was used to compare marker levels between rotator types.
Main Results:
- Significant BTG release occurred on all rotators after 24 hours.
- LDH discharge was significant only with elliptical rotation after 24 hours.
- Elliptical rotation showed significantly higher BTG and LDH levels compared to platform and circular rotation.
Conclusions:
- Platform and circular rotators result in less in vitro platelet activation and damage.
- Elliptical agitation leads to greater platelet activation and lysis.
- Platform and circular rotators are preferable for maintaining platelet concentrate quality during storage.