Spot blot: a hybridization assay for specific DNA sequences in multiple samples
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Updated: Oct 8, 2026

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Published on: August 6, 2011
Abstract:
Small aliquots of DNA-containing samples from gradient or column fractions are spotted onto agarose plates containing ethidium bromide, and the DNA is visualized qualitatively. After it is overlaid with a thin agarose cover, the DNA is denatured and transferred to nitrocellulose filters for hybridization. This method, called the "spot-blot assay," is particularly convenient when dealing with large numbers of samples.
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