Hoechst fluorescence pattern of heterochromatin in three closely related members of Drosophila

Heterochromatin distribution in metaphase chromosomes of three morphologically and karyologically similar members of Drosophila, D. s.sulfurigaster, D. s.bilimbata and D. pallidifrons was studied by the Hoechst 33258 staining method. Comparison showed remarkable uniformity of H-heterochromatin among the three members except for in their Y-chromosomes. On the basis of these observations phylogenetic relationships are discussed.