A simple method for the determination of riboflavin in human milk

A fluorometric method for the determination of total riboflavin in human milk is described. Flavin-adenine dinucleotide (FAD) is hydrolyzed to riboflavin by enzyme treatment. Then the fluorescence of total riboflavin is measured in the aqueous phase at pH 4.5. The coefficient of variation was found to be 2.48% (n = 58). The procedure is simple, reproducible and allows the simultaneous analysis of a considerable number of samples. The recoveries are in the range of 95.8-100.8%.