Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The correlation of light and electron microscope observations.

W T Perrie, D M Webb

    Journal of Microscopy
    |April 1, 1983
    PubMed
    Summary

    This study introduces a new method for electron microscopy, enabling precise ultrastructural analysis of specific tissue areas identified via light microscopy. This technique facilitates rapid localization and detailed examination of microscopic features in biological samples.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Restriction fragment length polymorphism and allozyme linkage map of Cuphea lanceolata.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
    Same author

    Genetic mapping of soybean cyst nematode race-3 resistance loci in the soybean PI 437.654.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
    Same author

    Clinical and statistical assessment of suicidal ideation and behavior in pharmaceutical trials.

    Clinical pharmacology and therapeutics·2011
    Same author

    Endemic avian toxoplasmosis on a farm in Illinois: clinical disease, diagnosis, biologic and genetic characteristics of Toxoplasma gondii isolates from chickens (Gallus domesticus), and a goose (Anser anser).

    Veterinary parasitology·2007
    Same author

    FoxP2 in song-learning birds and vocal-learning mammals.

    The Journal of heredity·2004
    Same author

    Fatal visceral and neural sarcocystosis in dogs.

    Journal of comparative pathology·1999

    Area of Science:

    • Histology and Ultrastructural Pathology
    • Microscopy Techniques

    Background:

    • Investigating specific tissue features at the ultrastructural level requires precise localization.
    • Traditional methods can be cumbersome for targeting small areas within large tissue sections.

    Purpose of the Study:

    • To present a novel technique for correlating light microscopy findings with electron microscopy.
    • To enable targeted ultrastructural analysis of specific regions within tissue sections.

    Main Methods:

    • Tissue sections on glass slides are processed for light microscopy.
    • Sections are embedded in epoxy resin, allowing for trimming under light microscopic guidance.
    • Semi-thin and ultra-thin sections are prepared for light and electron microscopy, respectively.

    Main Results:

    • The technique allows continuous observation of the target area during block trimming.
    • Semi-thin sections retain light microscopic staining for initial identification.
    • Ultra-thin sections provide high-resolution ultrastructural detail.

    Conclusions:

    • This method offers efficient and accurate localization of specific tissue areas for ultrastructural study.
    • It is particularly useful for examining inflammatory lesions, such as in polymyositis, at the ultrastructural level.

    Related Experiment Videos