Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new gene in E. coli RNA synthesis.

H H Liebke, J F Speyer

    Molecular & General Genetics : MGG
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    A novel Escherichia coli mutant stops RNA and protein synthesis at high temperatures. This temperature-sensitive mutant fails to produce guanine polyphosphate compounds, impacting bacterial growth and RNA regulation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Physiological effects of the fructose-1,6-diphosphate aldolase ts8 mutation on stable RNA synthesis in Escherichia coli.

    Journal of bacteriology·1991
    Same author

    Multi-sample electroporation.

    BioTechniques·1990
    Same author

    A simple and effective electroporation apparatus.

    BioTechniques·1990
    Same author

    Multiple SecA protein isoforms in Escherichia coli.

    Journal of bacteriology·1987
    Same author

    Interaction of an antimutator gene with DNA repair pathways in Escherichia coli K-12.

    Molecular & general genetics : MGG·1985
    Same author

    A conditional antimutator in E. coli.

    Molecular & general genetics : MGG·1977
    Same journal

    A multigene family in Calothrix sp. PCC 7601 encodes phycocyanin, the major component of the cyanobacterial light-harvesting antenna

    Molecular & general genetics : MGG·2017
    Same journal

    Mutants of Schizosaccharomyces pombe which sporulate in the haploid state.

    Molecular & general genetics : MGG·2015
    Same journal

    The chloroplast genomes of conifers lack one of the rRNA-encoding inverted repeats.

    Molecular & general genetics : MGG·2014
    Same journal

    Multiple mutant analysis of recombination in yeast.

    Molecular & general genetics : MGG·2012
    Same journal

    Intergenic suppression of spoO phenotypes by the Bacillus subtilis mutation rvtA.

    Molecular & general genetics : MGG·2010
    Same journal

    Expression of the seqA gene is negatively modulated by the HU protein in Escherichia coli.

    Molecular & general genetics : MGG·2001
    See all related articles

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Escherichia coli is a model organism for studying bacterial gene expression.
    • Regulation of stable RNA synthesis is crucial for bacterial growth and adaptation.
    • Temperature-sensitive mutants are valuable tools for dissecting cellular processes.

    Purpose of the Study:

    • To characterize a newly identified temperature-sensitive mutant of Escherichia coli.
    • To investigate the molecular basis of the temperature sensitivity affecting RNA and protein synthesis.
    • To identify the genetic locus responsible for the observed phenotype.

    Main Methods:

    • Isolation and characterization of a spontaneous temperature-sensitive mutant.
    • Analysis of stable RNA synthesis and degradation at permissive and non-permissive temperatures.

    Related Experiment Videos

  • Assay of guanine polyphosphate (ppGpp and pppGpp) synthesis.
  • Growth studies in various media at different temperatures.
  • Genetic mapping of the temperature-sensitive mutation.

    • Main Results:

    • The mutant ceases stable RNA and protein synthesis upon a shift from 30°C to 42°C.
    • Stable RNA species are not preferentially degraded in the mutant at the non-permissive temperature.
    • Synthesis of guanine polyphosphate compounds (ppGpp and pppGpp) is abolished at 42°C.
    • The mutant exhibits impaired growth at 42°C in diverse media.
    • The mutation maps to a novel locus affecting RNA synthesis in E. coli.

    Conclusions:

    • A new temperature-sensitive locus regulating RNA synthesis in Escherichia coli has been identified.
    • The mutation disrupts the production of guanine polyphosphates, essential for bacterial growth.
    • This mutant provides a new tool for studying RNA synthesis regulation in bacteria.