[Isolation of vital human sperm from seminal plasma following cryopreservation]

In the present paper the conditions of separation of cryopreserved spermatozoa from seminal plasma were varied. The optimum seems to be at 300 g and 5 to 7 min of centrifugation. The effect of separation was approximately estimated by means of determination of concentration, motility, morphology, and supravital staining of spermatozoa. The concentration of seminal plasma was reduced to less than one per cent (use of a radioactive marker). After the separation the activity of free acrosin reached 64 p.c. of the total acrosin. The process of cryopreservation and separation reduced the ouabain-sensitive netto-uptake of K+ of spermatozoa by nearly 50 p.c.