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The relationship between immunization and circulating antigen-specific plaque-forming cells.

R J Falkoff, L P Zhu, A S Fauci

    Cellular Immunology
    |June 1, 1983
    PubMed
    Summary

    Antibody-producing cells appear in circulation before a rise in serum antibodies after tetanus toxoid (TT) booster immunization. This indicates specific B-cell activation and correlates with overall antibody synthesis.

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    Area of Science:

    • Immunology
    • Cellular Biology

    Background:

    • Booster immunizations with recall antigens like tetanus toxoid (TT) elicit specific immune responses.
    • Understanding the kinetics of B-cell activation and antibody production is crucial for vaccine development.

    Purpose of the Study:

    • To investigate the relationship between circulating antibody-producing cells and serum antibody titers after TT booster immunization.
    • To characterize the specificity and kinetics of B-cell activation in response to TT.

    Main Methods:

    • Hemolytic plaque assay using TT-coated sheep red blood cells to detect antibody-producing cells (PFC).
    • Symmetric inhibition studies to confirm antigen specificity.
    • Staphylococcal protein A reverse hemolytic plaque assay to assess total Ig-secreting cells.

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    Main Results:

    • Plaque-forming cells (PFC) specific for TT were detected in circulation post-immunization.
    • Peak PFC numbers occurred between days 6-8, preceding the rise in serum IgG anti-TT titers.
    • No evidence of polyclonal B-cell activation was observed.

    Conclusions:

    • B-cell activation upon boosting with TT is highly specific.
    • The appearance of circulating activated B cells correlates with systemic IgG anti-TT synthesis.