Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Capillary endothelial cell cultures: phenotypic modulation by matrix components.

J A Madri, S K Williams

    The Journal of Cell Biology
    |July 1, 1983
    PubMed
    Summary

    Connective tissue components regulate capillary endothelial cell behavior. Different collagen types influence cell proliferation, structure formation, and migration, offering insights into angiogenesis and tissue repair.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Uncontrolled blood pressure and risk of sleep apnea among blacks: findings from the Metabolic Syndrome Outcome (MetSO) study.

    Journal of human hypertension·2015
    Same author

    Survey of management of common iliac artery aneurysms by members of the Vascular Society of Great Britain and Ireland.

    Annals of the Royal College of Surgeons of England·2014
    Same author

    Effects of packaging systems and fat concentrations on microbiology, sensory and physical properties of ground beef stored at 4±1°C for 25 days.

    Meat science·2014
    Same author

    Quantification of hepatic FOXP3+ T-lymphocytes in HIV/hepatitis C coinfection.

    Journal of viral hepatitis·2014
    Same author

    Prenatal and gestational cocaine exposure: Effects on the oxytocin system and social behavior with implications for addiction.

    Pharmacology, biochemistry, and behavior·2013
    Same author

    Generation of a functional liver tissue mimic using adipose stromal vascular fraction cell-derived vasculatures.

    Scientific reports·2013

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Extracellular Matrix Research

    Background:

    • Capillary endothelial cells (CECs) are crucial for blood vessel formation.
    • The extracellular matrix (ECM) influences cell behavior and tissue development.
    • Understanding CECs' interaction with ECM components is vital for regenerative medicine.

    Purpose of the Study:

    • To investigate the role of interstitial and basement membrane collagens in regulating CEC phenotype.
    • To explore CEC behavior and differentiation on various collagen substrata and amnionic membranes.
    • To establish in vitro culture systems for studying organized CECs.

    Main Methods:

    • Isolation and culture of rat epididymal fat pad CECs.
    • Culturing cells on interstitial collagens, basement membrane collagens, and acellular amnionic membranes.
    • Assessing cell proliferation, aggregation, tubelike structure formation, migration, and ECM constituent expression using immunoperoxidase and ELISA methods.

    Main Results:

    • CECs proliferated and formed cell layers on interstitial collagens, with some tubelike structures.
    • CECs aggregated and formed tubelike structures without proliferation on basement membrane collagens.
    • Cells cultured on basement membrane expressed more basement membrane constituents.
    • CECs adhered, spread, and formed tubes on the amnion's basement membrane side but did not migrate through it.
    • CECs proliferated, migrated into the stroma, and formed tubes on the amnion's stromal side.

    Conclusions:

    • Connective tissue components, particularly collagens, significantly regulate CEC phenotypic expression in vitro.
    • ECM components likely play similar roles in vivo during angiogenesis and tissue repair.
    • The developed culture systems are valuable for studying differentiated and organized CECs.

    Related Experiment Videos