Related Experiment Video
Updated: Aug 11, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
[Effect of deuteration on the gramicidin-sensitive electron-transport reactions in chloroplasts]
Abstract:
The uncoupling effects of gramicidine and NH4Cl on the light-induced electron transport were studied in isolated pea chloroplasts placed in H2O- or D2O-media. Gramicidine at 10(-6) M had no uncoupling effect on the rate of ferricyanide-supported O2 evolution in a D2O-medium, but enhanced the ferricyanide reduction in a H2O-medium and completely inhibited the light-induced alkalinization both in H2O- and D2O-suspended chloroplasts. The rate of electron transport with methylviologen as an acceptor was gramicidine-sensitive (uncoupling) both in preparations with H2O and D2O. In D2O-suspended chloroplasts the phosphorylation activity was retained as well as control of ferricyanide-dependent electron flow by ADP--phosphate and NH4Cl. It was assumed that some electron carriers on the inner surface of thylakoid membranes become inaccessible to ferricyanide upon deuteration.
More Related Videos
09:00Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
Published on: April 16, 2018
07:58Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation
Published on: January 12, 2024
Related Concept Videos
The Electron Transport Chain
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q in...
¹H NMR of Labile Protons: Deuterium (²H) Substitution
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
Electron Transport Chain Components
Microbial Bioremediation of Pesticides