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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
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Published on: January 29, 2014

[The demonstration of HL-A antigens on thrombocytes under various conditions]

F W Bube, D Seegers, E Siebel

    Folia Haematologica (Leipzig, Germany : 1928)
    |January 1, 1976
    PubMed

    Platelets of healthy test persons were gained according to the method of Aster (avital cells) and according to the procedure of BUBE and GMURZYNSKI (vital cells). Because of their tendency towards aggregation vital cells do bind any HL-A antibodies and whereas avital cells will fix antibodies and complement depending on the height of the titre. Thrombocytes stored in the own serum will respond less to the micro-C'-fixation test at + 4 degrees C as well as--196 degrees C, whereas storage in NaN3/NaCl and under the conditions of the own serum will reveal no differences up to--196 degrees C in each case.

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