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Related Experiment Videos

Pancreatic structure and function in the immature reserpinized rat.

S L Werlin, J M Harb, J Stefaniak

    Experimental and Molecular Pathology
    |August 1, 1983
    PubMed
    Summary

    Reserpine treatment in immature rats, mimicking cystic fibrosis, caused pancreatic changes. Prenatal and neonatal exposure induced hypoplasia and abnormal zymogen granules, suggesting a suitable animal model.

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    Area of Science:

    • Pancreatology
    • Developmental Biology
    • Pharmacology

    Background:

    • Cystic fibrosis (CF) in infants is associated with pancreatic exocrine insufficiency.
    • Current animal models for studying CF-related pancreatic issues in neonates are limited.
    • Reserpine is known to affect adult rat pancreas; its effects on immature pancreas are less understood.

    Purpose of the Study:

    • To evaluate the suitability of reserpine-induced pancreatic changes in immature rats as a model for infant cystic fibrosis.
    • To investigate the effects of prenatal and neonatal reserpine administration on pancreatic development and function.

    Main Methods:

    • Immature rats were administered reserpine during fetal development or as neonates, using lower doses than previously reported to avoid lethality.
    • Pancreatic tissue was analyzed at various time points (fetal, 1-day-old, 7-day-old, 14-day-old, 21-day-old) for morphological and biochemical changes.

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  • Electron microscopy was employed to examine pancreatic acinar cell structure and zymogen granules.

    • Main Results:

    • Prenatal reserpine exposure led to fetal pancreatic hypoplasia with elevated chymotrypsinogen at 1 day, followed by hyperplasia at 7 days.
    • Neonatal reserpine exposure resulted in pancreatic hypoplasia and decreased measured parameters at 7 days.
    • Electron microscopy revealed acinar cell degeneration, abnormal zymogen granules, and altered granule content/size in treated rats compared to controls.
    • Progressive recovery was observed in both groups within 2 weeks post-treatment.

    Conclusions:

    • Prenatal and neonatal reserpinization in rats induces pancreatic alterations resembling those observed in cystic fibrosis.
    • This reserpine-induced model in immature rats shows potential for studying pancreatic exocrine insufficiency in infant cystic fibrosis.
    • Further research is warranted to fully characterize this model and its applicability to CF pathogenesis.