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Related Experiment Videos

Antigen-induced human T cell help. Precursor frequency, radiation sensitivity, and allogeneic effects.

H C Lane, G Whalen, A S Fauci

    The Journal of Clinical Investigation
    |August 1, 1983
    PubMed
    Summary

    Human B cell responses differ significantly when stimulated by antigens versus mitogens. These differences impact T cell help and allogeneic effects, crucial for understanding antibody production in health and disease.

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    Area of Science:

    • Immunology
    • Cellular Biology
    • Human B cell activation

    Background:

    • In vitro human B lymphocyte responses vary between soluble antigen and mitogen stimulation.
    • Understanding these differences is key to dissecting specific vs. nonspecific antibody production.

    Purpose of the Study:

    • Analyze differences in T and B cell precursor frequencies and T cell radiation sensitivity between antigen- and mitogen-induced responses.
    • Investigate allogeneic effects in human antigen-specific B cell responses.
    • Clarify the relationship between specificity and nonspecificity in antibody production.

    Main Methods:

    • Comparative analysis of in vitro human B lymphocyte responses.
    • Assessment of T and B cell precursor frequencies.
    • Evaluation of T cell radiation sensitivity.

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  • Delineation of allogeneic effects in antigen-specific systems.

    • Main Results:

    • Significant differences observed in T cell precursor frequencies and radiation sensitivity between antigen- and mitogen-induced systems.
    • B cell precursor frequencies were similar in both systems.
    • Marked positive and negative allogeneic effects were identified in antigen-specific B cell responses.

    Conclusions:

    • Antigen-specific and mitogen-induced activation pathways for human B cells exhibit distinct characteristics.
    • Allogeneic effects must be considered in studies of human T cell-B cell collaboration.
    • These findings are vital for understanding antibody production in normal and pathological conditions.