Construction of composite slides
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Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
Published on: April 27, 2012
The Journal of Prosthetic Dentistry
|June 1, 1983
Abstract:
This article describes a method for constructing composite slides without destroying the original slide. A conventional slide projector, rear-projector screen, modified 35 mm camera, and slide-duplicating film are required for this technique.
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Fixation and Sectioning
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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When applying the method of superposition, each type of load—whether...
When applying the method of superposition, each type of load—whether...
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Design Example: Designing Water Slide
When designing a water slide, controlling the speed of water flow is crucial for rider safety while maintaining an exciting experience. As water flows down the slide, gravity causes it to accelerate, with its speed at the bottom depending on the height from which it starts. The higher the slide, the more potential energy the water has at the top, which is converted into kinetic energy as it descends, increasing its speed.
Bernoulli's principle determines the water's velocity along the slide.
Bernoulli's principle determines the water's velocity along the slide.

