Suppression of photo-induced sporulation in Trichoderma viride by inhibitors

The mycelium of Trichoderma viride grown in the dark under submerged conditions and transferred to membrane filters sporulated only after photoinduction. The optimum photoinduction of sporulation was reached when applying daylight for 3 min and near ultraviolet radiation (355 nm) for 10 to 30 sec. After the photoinduction probounced synthesis of DNA, RNA and protein was observed. The photoinduced sporulation was partially or fully inhibited in the presence of phenethyl alcohol, actinomycin D, 5-fluorouracil, cycloheximide and ethidium bromide. The same inhibitors blocked also the photoinduced sporulation of surface growing colonies of Trichoderma viride. Various inhibitiors of synthesis of nucleic acids and protein, inhibitors impairing the function of membranes and certain other compounds were also effective.