[Methods for cytologic and histologic determination of phospholipids in lung tissue]

The demonstration of lipids by light microscopy is one of the most difficult parts of histochemistry. Phospholipids of the surfactant system are a very important part of lung metabolism, but for morphologists not easy to detect. Basing on own experiences, a view is given about different methods for demonstration of phospholipids, in particular of the surfactant system by light microscopy and fluorescent microscopy.