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Detection of specific murine anti-tumour immunity and serum factors by the indirect E-rosette augmentation assay
The spontaneous interaction of sheep erythrocytes and human lymphocytes results in the formation of aggregates known as E-rosettes. The antigen reactivity of murine T-lymphocytes can be detected indirectly by the production of a lymphokine that augments the E-rosette-forming ability of human lymphocytes. An indirect E-rosette augmentation (ERA) assay was used to study cellular reactivity and tumour-related serum reactions in mice bearing methylcholanthrene (MCA)-induced tumours. The assay was used to demonstrate the antigenic specificity of two tumours and to detect blocking factors in tumour-bearer serum. Blocking factors were shown to be tumour-specific and to act on lymphocytes at an early stage before exposure to antigen.
The spontaneous interaction of sheep erythrocytes and human lymphocytes results in the formation of aggregates known as E-rosettes. The antigen reactivity of murine T-lymphocytes can be detected indirectly by the production of a lymphokine that augments the E-rosette-forming ability of human lymphocytes. An indirect E-rosette augmentation (ERA) assay was used to study cellular reactivity and tumour-related serum reactions in mice bearing methylcholanthrene (MCA)-induced tumours. The assay was used to demonstrate the antigenic specificity of two tumours and to detect blocking factors in tumour-bearer serum. Blocking factors were shown to be tumour-specific and to act on lymphocytes at an early stage before exposure to antigen.