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Description of a new Qa antigenic specificity, "Qa-m9," whose expression is under complex genetic control
Journal of Immunology (Baltimore, Md. : 1950)
|September 1, 1983
Summary
The study reveals that the expression of the Qa-m9 antigen is controlled by three genes, influencing its diversity. This multi-gene control allows for the generation of new antigens through post-translational modifications.
Area of Science:
- Immunogenetics
- Molecular immunology
Background:
- The Qa-m9 antigen specificity was defined using a monoclonal antibody.
- Evidence suggests a three-gene system controls Qa-m9 expression.
Purpose of the Study:
- To elucidate the genetic control of Qa-m9 antigen expression.
- To investigate the molecular basis of Qa-m9 antigen diversity.
Main Methods:
- Utilized H-2 linkage analysis (congenic strains, Rl lines).
- Employed differential reactivity assays (B6.K1, B6.K2).
- Conducted biochemical analysis to identify protein chains (39,000 Mr and 12,000 Mr).
Main Results:
- Identified three genes controlling Qa-m9: an H-2 linked Qa gene, an H-3 linked gene (likely beta-2 microglobulin), and an H-2D linked gene regulating antigen quantity.
- Qa-m9 is expressed on T and B cells, some bone marrow cells, but not thymocytes.
- Antigen determinant is likely combinatorial or conformational, requiring specific heavy and light chain association.
Conclusions:
- Demonstrated multi-gene control over antigen expression.
- Showcased potential for generating novel antigens via post-translational mechanisms.