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Published on: January 6, 2017

Effects of hydrocortisone and cholecystokinin-octapeptide on neonatal rat pancreas

S L Werlin, J Stefaniak

    Journal of Pediatric Gastroenterology and Nutrition
    |January 1, 1982
    PubMed

    Insights

    Newborn rats treated with hydrocortisone and cholecystokinin-octapeptide (CCK-8) showed increased pancreatic growth and enzyme activity. However, hydrocortisone later reduced pancreatic amylase secretion, while CCK-8 enhanced it.

    Area of Science:

    • Endocrinology
    • Developmental Biology
    • Gastroenterology

    Background:

    • The development and function of the pancreas are crucial for digestion.
    • Hormonal regulation plays a significant role in organ development during early life.

    Purpose of the Study:

    • To investigate the effects of hydrocortisone and cholecystokinin-octapeptide (CCK-8) on the growth and secretory capacity of the newborn rat pancreas.
    • To understand the distinct roles of glucocorticoids and CCK-8 in pancreatic development.

    Main Methods:

    • Newborn rats were injected with hydrocortisone or CCK-8 from days 1-5 of life.
    • Pancreatic weight, protein, and DNA content were measured.
    • Specific activities of digestive enzymes (amylase, chymotrypsinogen, lipase) were assessed.
    • Pancreatic amylase secretion in response to carbachol and basal secretion were measured at 7 days of age.

    Main Results:

    • Both hydrocortisone and CCK-8 significantly increased pancreatic weight, protein, and DNA content.
    • Specific activities of amylase, chymotrypsinogen, and lipase were elevated by both agents.
    • Hydrocortisone administration at 7 days decreased the pancreas's ability to secrete amylase in response to carbachol.
    • CCK-8 administration at 7 days increased basal pancreatic secretion.

    Conclusions:

    • Glucocorticoids and cholecystokinin are key regulators of newborn rat pancreatic growth.
    • These hormones differentially influence the secretory capacity of the developing pancreas.
    • Early-life hormonal exposure has lasting effects on pancreatic function.

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