Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human alpha-fetoprotein-fatty acid interaction.

C Aussel, R Masseyeff

    Biochemical and Biophysical Research Communications
    |August 30, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Diagnostic and prognostic value of brain natriuretic peptide (BNP) concentrations in very elderly heart disease patients: specific geriatric cut-off and impacts of age, gender, renal dysfunction, and nutritional status.

    Archives of gerontology and geriatrics·2010
    Same author

    Commonly used estimates of creatinine clearance are inadequate for the very elderly.

    The journal of nutrition, health & aging·2009
    Same author

    Splanchnic sequestration of amino acids in aged rats: in vivo and ex vivo experiments using a model of isolated perfused liver.

    American journal of physiology. Regulatory, integrative and comparative physiology·2007
    Same author

    Is biolectrical impedance analysis a tool at bedside, during heat waves to assist geriatricians with discriminative diagnosis of hypertonic dehydration?

    The journal of nutrition, health & aging·2006
    Same author

    Use of ornithine alpha-ketoglutarate in clinical nutrition of elderly patients.

    Nutrition (Burbank, Los Angeles County, Calif.)·2003
    Same author

    [Evaluating an internal quality control procedure: application to multidimensional control].

    Annales de biologie clinique·2002
    Same journal

    The plasma membrane H<sup>+</sup>-ATPase OSA2 negatively regulates salt tolerance in rice seedlings.

    Biochemical and biophysical research communications·2026
    Same journal

    MiR-425-5p modulation of CREB1 affects inflammatory response and motor recovery after spinal cord injury.

    Biochemical and biophysical research communications·2026
    Same journal

    Xanthoangelol, a chalcone from Angelica keiskei, induces apoptosis in cervical cancer cell lines via the oxidative stress-mediated activation of caspase-8 and caspase-9.

    Biochemical and biophysical research communications·2026
    Same journal

    Suchilactone ameliorates podocyte injury in membranous nephropathy by modulating the JNK/p38 MAPK pathway in vitro.

    Biochemical and biophysical research communications·2026
    Same journal

    Palmitic acid-induced metabolic stress alters differentiation-associated gene expression in human ameloblast-like cells.

    Biochemical and biophysical research communications·2026
    Same journal

    A transferrin receptor-based vector enables robust Type-II membrane protein display on mammalian cells.

    Biochemical and biophysical research communications·2026
    See all related articles

    Human alpha-fetoprotein (AFP) binds long-chain polyunsaturated fatty acids with high affinity. This specific interaction highlights AFP's role in fatty acid transport and binding, excluding prostaglandins.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Protein-Ligand Interactions

    Background:

    • Human alpha-fetoprotein (AFP) is a serum protein with largely unknown functions.
    • Understanding AFP's binding properties is crucial for elucidating its physiological roles.

    Purpose of the Study:

    • To investigate the binding specificity of human alpha-fetoprotein (AFP) for various fatty acids.
    • To characterize the affinity and binding site characteristics for arachidonic acid.

    Main Methods:

    • Competitive binding assays using radiolabeled arachidonic acid.
    • Testing of thirty different fatty acids to assess binding interactions with AFP.

    Main Results:

    • Human AFP exhibits high-affinity binding (Ka = 10(7)M-1) to arachidonic acid, with a limited number of binding sites (NS = 3).

    Related Experiment Videos

  • The interaction demonstrates significant specificity, with only polyunsaturated long-chain fatty acids showing high-affinity binding.
  • Metabolic products of arachidonic acid, such as prostaglandins, did not bind to AFP.

    • Conclusions:

    • Human AFP possesses a specific binding site for polyunsaturated long-chain fatty acids, particularly arachidonic acid.
    • This specificity suggests a potential role for AFP in the transport or regulation of these fatty acids.
    • AFP does not appear to bind prostaglandins, differentiating its function from other arachidonic acid-binding proteins.