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Granular and globular acid alpha-naphthyl-acetate-esterase staining pattern of resting and mitogen-activated murine
Available methods for the identification of T-lymphocytes in tissue sections and in single cell suspensions are often tedious and require a lot of manipulation. Here we confirm previous findings that nonspecific alpha-naphthyl-acetate esterase (ANAE) is a reliable method for the identification of resting thymus-derived lymphocytes but not for concanavalin-A-stimulated lymphocytes, as a small proportion of lipopolysaccharide-stimulated lymphoblasts exhibit ANAE activity as well. We have also shown that there is a relationship between the globular staining pattern and the degree of cell activation. Finally, we noted that prolonged incubation of lymphocytes at 37 degrees C reduces the number of ANAE-positive cells.
Available methods for the identification of T-lymphocytes in tissue sections and in single cell suspensions are often tedious and require a lot of manipulation. Here we confirm previous findings that nonspecific alpha-naphthyl-acetate esterase (ANAE) is a reliable method for the identification of resting thymus-derived lymphocytes but not for concanavalin-A-stimulated lymphocytes, as a small proportion of lipopolysaccharide-stimulated lymphoblasts exhibit ANAE activity as well. We have also shown that there is a relationship between the globular staining pattern and the degree of cell activation. Finally, we noted that prolonged incubation of lymphocytes at 37 degrees C reduces the number of ANAE-positive cells.