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Histamine releasers and rat mast cells
International Archives of Allergy and Applied Immunology
|January 1, 1983
Summary
Histamine release from rat mast cells varies significantly between in vitro and in vivo conditions. Experimental context is crucial when comparing the efficacy of different histamine-releasing agents.
Area of Science:
- Immunology
- Pharmacology
Background:
- Mast cells are key players in allergic reactions, releasing histamine.
- Histamine release can be triggered by various agents, including allergens and chemical compounds.
Purpose of the Study:
- To compare histamine release from rat tissues and isolated mast cells induced by different agents.
- To evaluate the in vivo versus in vitro efficacy of various histamine releasers.
Main Methods:
- Comparing histamine release from rat tissues and isolated peritoneal mast cells.
- Utilizing various histamine-releasing agents: antigen, concanavalin A, dextran, compound 48/80, A 23187, phosphatidic acid, and chlortetracycline.
- Administering agents intradermally and subcutaneously in vivo, and assessing release in vitro.
Main Results:
- Most agents showed higher activity than dextran in vitro.
- In vivo (intradermal/subcutaneous) administration revealed different activity patterns.
- Calcium ionophores (A 23187, chlortetracycline) and phosphatidic acid showed minimal histamine release in vivo.
Conclusions:
- The experimental conditions significantly impact the observed histamine-releasing activity of agents.
- In vitro results do not always predict in vivo histamine release efficacy.
- Stating experimental conditions is essential for accurate comparisons of histamine releasers.