Simultaneous visualization of Nuclear yellow and iron-dextran complex for demonstration of branched neurons by...

Retrogradely transported iron-dextran complex and Nuclear yellow could be demonstrated simultaneously on the same sections of rat CNS. Using combined bright-field and ultraviolet illuminations, double labeled neurons were observed. They exhibited a fluorescent nucleus and cytoplasmic Prussian blue granules. Thus, the combination of these two tracers appeared to be a convenient method for the anatomical demonstration of collaterals.