Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence for human argininosuccinate synthetase cDNA.

H G Bock, T S Su, W E O'Brien

    Nucleic Acids Research
    |September 24, 1983
    PubMed
    Summary

    Researchers sequenced human argininosuccinate synthetase cDNA, revealing its genetic code. This study explores potential regulatory mechanisms for enzyme production, including arginine-mediated repression.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Erratum to: The Cognitive and Behavioral Phenotypes of Individuals with CHRNA7 Duplications.

    Journal of autism and developmental disorders·2017
    Same author

    Further delineation of a rare recessive encephalomyopathy linked to mutations in GFER thanks to data sharing of whole exome sequencing data.

    Clinical genetics·2017
    Same author

    The Cognitive and Behavioral Phenotypes of Individuals with CHRNA7 Duplications.

    Journal of autism and developmental disorders·2016
    Same author

    Identification of incestuous parental relationships by SNP-based DNA microarrays.

    Lancet (London, England)·2011
    Same author

    22q13.3 deletion syndrome: clinical and molecular analysis using array CGH.

    American journal of medical genetics. Part A·2010
    Same author

    Microdeletion 15q13.3: a locus with incomplete penetrance for autism, mental retardation, and psychiatric disorders.

    Journal of medical genetics·2009

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Genetics

    Background:

    • Argininosuccinate synthetase (ASS) is a key enzyme in the urea cycle.
    • Understanding ASS gene regulation is crucial for metabolic disorders.

    Purpose of the Study:

    • To determine the nucleotide sequence of human argininosuccinate synthetase cDNA.
    • To investigate potential regulatory mechanisms of ASS gene expression.

    Main Methods:

    • Analysis of six human argininosuccinate synthetase cDNA clones.
    • Nucleotide sequencing and sequence analysis.

    Main Results:

    • A 1623-nucleotide sequence was determined, containing a 1236-nucleotide open reading frame encoding a 46,434-dalton protein.
    • Potential cloning artifacts and single base variations were identified.
    • Three tandem arginine codons were found in the 5' untranslated region.

    Conclusions:

    • The determined cDNA sequence provides a basis for further functional studies of human argininosuccinate synthetase.
    • The presence of arginine codons suggests a possible mechanism for arginine-mediated translational regulation.

    Related Experiment Videos