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Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein

R A Padgett, S M Mount, J A Steitz

    Cell
    |November 1, 1983
    PubMed

    Insights

    Human autoimmune sera and a mouse antibody targeting U1 ribonucleoprotein particles (RNP) inhibited messenger RNA (mRNA) splicing in vitro. This suggests U1 RNP is essential for mRNA precursor splicing.

    Area of Science:

    • Molecular Biology
    • Immunology
    • Virology

    Background:

    • Messenger RNA (mRNA) splicing is a critical post-transcriptional modification.
    • Small ribonucleoprotein particles (RNPs) are implicated in various cellular processes, including RNA processing.

    Purpose of the Study:

    • To investigate the role of specific small RNPs in mRNA splicing.
    • To determine if antibodies against RNPs can inhibit mRNA splicing.

    Main Methods:

    • In vitro splicing assay using adenovirus late RNA.
    • Testing inhibition by mouse monoclonal antibodies and human autoimmune sera against U1 RNP, Sm RNPs, U2 RNP, La, and Ro RNPs.

    Main Results:

    • Sera reacting with U1 RNP specifically inhibited mRNA splicing.
    • A mouse monoclonal antibody against Sm RNPs also inhibited splicing.
    • Antisera against U2 RNP, La, or Ro RNPs did not affect splicing.

    Conclusions:

    • U1 RNP is essential for the splicing of mRNA precursors.
    • Antibodies targeting U1 RNP can disrupt the mRNA splicing process.

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