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Modification of lipopolysaccharide non-responder cells in low cell density culture
Lipopolysaccharide (LPS) fails to induce polyclonal antibody responses in LPS non-responder C3H/HeJ mice in vivo and also in in vitro cultures of 10(7) cells/ml of C3H/HeJ spleen cells. However, this study shows that LPS elicits polyclonal antibody synthesis in low cell density cultures of C3H/HeJ cells by activation of a translational process without an increase in total RNA and messenger RNA synthesis. Polyclonal antibody synthesis by LPS non-responder cells in low cell density culture was significantly increased by LPS in either serum-free medium or medium containing serum. LPS-induced antibody production by LPS non-responder cells in low cell density was not due to the relative elevation of LPS concentrations.
Lipopolysaccharide (LPS) fails to induce polyclonal antibody responses in LPS non-responder C3H/HeJ mice in vivo and also in in vitro cultures of 10(7) cells/ml of C3H/HeJ spleen cells. However, this study shows that LPS elicits polyclonal antibody synthesis in low cell density cultures of C3H/HeJ cells by activation of a translational process without an increase in total RNA and messenger RNA synthesis. Polyclonal antibody synthesis by LPS non-responder cells in low cell density culture was significantly increased by LPS in either serum-free medium or medium containing serum. LPS-induced antibody production by LPS non-responder cells in low cell density was not due to the relative elevation of LPS concentrations.