Affinity chromatographic quantitation of glycosylated hemoglobin in newborn infants

Levels of glycosylated hemoglobin (glyco Hb) were determined in 45 newborn infants by affinity chromatography on phenylboronate-agarose gel and found to correlate well with the results obtained by two chemical methods. However the percentages of Hb FIa+b and Hb FIc determined by Bio-Rex 70 chromatography did not correlate with the glyco Hb values. The glyco Hb levels in the newborns were significantly lower than in the adults. Rechromatography of the glyco and nonglyco Hb fractions on Bio-Rex 70 columns indicated that Hb FO-like glyco Hb (presumably formed by glycosylation of the alpha chain amino terminus and epsilon-amino groups of lysyl amino acids) constituted about 60% of the glyco Hb fraction, whereas both Hb FIc and Hb FIa+b components contained only small portions of the glyco Hb.