Expression of alpha subunit and luteinizing hormone beta genes in the ovine anterior pituitary. Estradiol suppresses accumulation of mRNAS for both alpha subunit and luteinizing hormone beta
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View abstract on PubMed
Summary
This summary is machine-generated.Estradiol significantly reduces messenger RNA (mRNA) for luteinizing hormone beta (LH beta) and alpha subunits in ewe pituitary glands. This estrogen effect impacts glycoprotein hormone production, suggesting regulation occurs before translation.
Area Of Science
- Endocrinology
- Molecular Biology
- Reproductive Science
Background
- Anterior pituitary gland produces crucial hormones like growth hormone, prolactin, and luteinizing hormone (LH).
- Glycoprotein hormones, such as LH, consist of alpha and beta subunits, with their synthesis regulated by various factors.
Purpose Of The Study
- To investigate the effect of estradiol on the mRNA concentrations of growth hormone, prolactin, alpha subunit, and luteinizing hormone beta (LH beta) in the ovine anterior pituitary.
- To determine if estrogen regulates the expression of genes encoding LH subunits at the pre-translational level.
Main Methods
- Bovine cDNA clones were used to quantify specific mRNA levels in pituitary glands from ovariectomized ewes.
- Ewes were either untreated or treated with estradiol for three weeks.
- Messenger RNA concentrations for growth hormone, prolactin, LH beta, and alpha subunit were measured.
Main Results
- Estradiol treatment caused a 98% decrease in LH beta mRNA and an 86% decrease in alpha subunit mRNA.
- Prolactin and growth hormone mRNA levels remained unchanged.
- Decreased subunit mRNA levels correlated with reduced pituitary and serum LH concentrations.
Conclusions
- Estrogen significantly downregulates the expression of genes encoding both the alpha and LH beta subunits, indicating pre-translational regulation.
- The substantial impact of estradiol on these subunit mRNAs suggests that glycoprotein hormone assembly may be influenced by factors beyond just beta subunit accumulation.
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