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Isolation of alloreactive CTL clones with cyclical changes in lytic activity
Journal of Immunology (Baltimore, Md. : 1950)
|November 1, 1983
Summary
Cytotoxic T-lymphocyte (CTL) clones showed varying lytic activity after stimulation with allogeneic cells or conditioned media. This modulation of CTL activity offers a model for studying immune responses in culture.
Area of Science:
- Immunology
- Cellular immunology
- T-cell biology
Background:
- Cytotoxic T-lymphocytes (CTLs) are crucial for adaptive immunity.
- CTL activity can be modulated by various stimuli.
- Understanding CTL modulation is key to developing immunotherapies.
Purpose of the Study:
- To investigate the changes in lytic activity of cloned CTLs.
- To explore the role of allogeneic stimulation and conditioned media in CTL modulation.
- To establish a model for studying CTL activity in vitro.
Main Methods:
- Isolation of H-2Dd specific CTL clones from mixed lymphocyte culture (MLC).
- Weekly stimulation of CTL clones with irradiated allogeneic cells and conditioned media.
- Assessment of CTL lytic activity against tumor and Con A blast target cells at different time points.
- Irradiation of CTLs before stimulation to assess its effect on activity.
Main Results:
- CTL clones exhibited peak lytic activity 2-3 days post-stimulation, with activity declining by days 5-7.
- Pre-stimulation irradiation of CTLs enhanced lytic activity on day 2.
- Conditioned media alone supported a dose-dependent increase in CTL activity.
- The observed changes mimicked the induction of lytic activity in long-term MLC.
Conclusions:
- Cloned CTL lines demonstrate dynamic changes in lytic activity upon stimulation.
- Conditioned media plays a significant role in modulating CTL activity.
- These CTL clones provide a valuable model for studying CTL activity modulation in culture.