Related Experiment Videos
Nondissociating cationic immune complexes can deposit in glomerular basement membrane
The Journal of Experimental Medicine
|November 1, 1983
Summary
This study demonstrates that intact immune complexes (IC) can deposit in the glomerular basement membrane (GBM). Covalently linked IC, when appropriately charged, crossed the GBM and deposited in subepithelial sites.
Area of Science:
- Immunology
- Nephrology
- Biochemistry
Background:
- The mechanism of immune complex (IC) deposition in the glomerular basement membrane (GBM) is debated.
- Key questions involve direct deposition of circulating IC versus in situ formation.
Purpose of the Study:
- To determine if intact IC can deposit directly in the GBM.
- To investigate the role of charge in IC deposition.
Main Methods:
- Covalently linked IC were created using toluene-2,4-diisocyanate (TDI), bovine gamma globulin (BGG), and rabbit anti-BGG antibody.
- IC were purified, and their net charge was chemically modified.
- Cationic IC were intravenously injected into mice.
Main Results:
- Antigen and antibody codeposited diffusely in the GBM of injected mice.
- Ultrastructural analysis revealed deposits in the laminae rarae and lamina densa.
- Intact IC crossed the GBM and deposited in subepithelial sites.
Conclusions:
- Intact immune complexes can deposit in the glomerular basement membrane.
- Charge plays a critical role in facilitating IC passage across the GBM.
- Size alone does not exclude intact IC deposition in subepithelial sites.