Transformation of human lung cells by 3-methylcholanthrene in vitro

Human fetal lung tissue was cultured on dead, sterile pigskin as a substrate and treated with 3-methylcholanthrene. Several cell lines were obtained. One of the lines produced a tumor in nude mouse and showed anchorage-independent growth in soft agar. The tumor cells were stained positive for keratin, indicating their epithelial origin.