Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extravascular diffusion in normal and neoplastic tissues.

L J Nugent, R K Jain

    Cancer Research
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Optimization of biolistic method for transient gene expression and production of agronomically useful transgenic Basmati rice plants.

    Plant cell reports·2013
    Same author

    Stimulatory effect of water stress on plant regeneration in aromatic Indica rice varieties.

    Plant cell reports·2013
    Same author

    Pharmacologic reperfusion therapy with indigenous tenecteplase in 15,222 patients with ST elevation myocardial infarction - the Indian Registry.

    Indian heart journal·2013
    Same author

    A highly efficient and compact long pulse Nd:YAG rod laser with 540 J of pulse energy for welding application.

    The Review of scientific instruments·2013
    Same author

    Tomato leaf curl New Delhi virus is Associated With Pumpkin Leaf Curl: A New Disease in Northern India.

    Indian journal of virology : an official organ of Indian Virological Society·2013
    Same author

    Editor's Note.

    Indian journal of virology : an official organ of Indian Virological Society·2013

    Extravascular transport of macromolecules in normal and tumor tissues was modeled using diffusion. Tumor tissue showed less hindrance to macromolecular transport compared to normal tissue, indicating differences in interstitial space and composition.

    Area of Science:

    • Biomedical Engineering
    • Pharmacokinetics
    • Tissue Engineering

    Background:

    • Understanding extravascular transport is crucial for drug delivery and tissue engineering.
    • Tumor microenvironments exhibit unique characteristics influencing macromolecular diffusion.
    • Rabbit ear chamber models provide in vivo insights into tissue transport dynamics.

    Purpose of the Study:

    • To investigate the extravascular transport of macromolecules in normal and tumor tissues.
    • To compare diffusion characteristics of various molecular sizes in different tissue types.
    • To elucidate the relationship between molecular size and diffusion coefficients in normal and cancerous tissues.

    Main Methods:

    • Utilized fluorescein isothiocyanate-conjugated bovine serum albumin and dextrans of varying molecular weights (Mr 19,400–71,800).

    Related Experiment Videos

  • Employed a rabbit ear chamber model to study transport in granulation tissue (normal) and VX2 carcinoma (tumor).
  • Applied a one-dimensional diffusion model and measured diffusion coefficients.

    • Main Results:

    • A one-dimensional diffusion model effectively described extravascular transport in both normal and tumor tissues.
    • Diffusion coefficients decreased with increasing molecular size, deviating from free diffusion in water.
    • Macromolecular transport was less hindered in tumor tissue than in normal tissue, correlating with tumor characteristics.

    Conclusions:

    • Tumor interstitial space and composition facilitate greater macromolecular transport compared to normal tissues.
    • The findings support the hypothesis of reduced glycosaminoglycans and larger interstitial spaces in tumors.
    • Diffusion coefficients for dextrans follow a power-law relationship with molecular weight in both normal and tumor tissues.