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Mouse monoclonal antibodies against rabbit b6 allotype and their use for characterization of light-chain

E Petit-Koskas, J Roland, A Benammar

    Molecular Immunology
    |November 1, 1983
    PubMed

    Mouse anti-allotypic hybridomas directed against different antigenic determinants of the b6 rabbit allotype have been raised. The fine specificity of these monoclonal antibodies (mAbs) was determined by radioimmunoassay and it was possible to classify them into three groups, each directed against distinct epitopes of the b6 allotype. Hare "b6" IgG were tested with anti-b6 mAb and no reaction was found indicating that the number of allotopes present on b6 molecules is greater than the three detected by the mAb. Comparative analysis by precipitation in gel of IgG from homozygous b6/b6 rabbits using mouse mAb and rabbit anti-b6 antibodies suggested that at least two categories of molecules can be discriminated. The observation of b6 subpopulations was confirmed by isolation of a minor subpopulation of IgG on a mouse monoclonal immunoadsorbent.

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