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Acquired B antigen: further studies using synthetic oligosaccharides
Revue Francaise De Transfusion Et Immuno-Hematologie
|September 1, 1983
Summary
Investigating acquired B red cells, this study found that the B-NH2 trisaccharide specifically inhibits "anti-acquired B" agglutinins, suggesting a shared structure between "B-like" and acquired B determinants, distinct from Tk antigens.
Area of Science:
- Immunology
- Biochemistry
- Glycobiology
Background:
- Acquired B red blood cell antigen presents a unique serological challenge.
- Understanding the molecular basis of acquired B antigen is crucial for transfusion medicine.
Purpose of the Study:
- To investigate the structural basis of acquired B red cell reactivity.
- To differentiate acquired B antigens from other related blood group antigens.
Main Methods:
- Agglutination inhibition assays using chemically synthesized trisaccharides.
- Testing inhibition with anti-A, anti-B, and anti-acquired B antisera.
- Investigating Tk-activated erythrocytes and BS-II lectin interactions.
Main Results:
- The B-NH2 trisaccharide specifically inhibited "anti-acquired B" agglutinins.
- The B-OAc trisaccharide did not inhibit anti-acquired B agglutinins.
- Tk-activated erythrocytes were inhibited by N-acetylglucosamine, not B-related trisaccharides.
Conclusions:
- The acquired B determinant shares a common structure with the B-like determinant, represented by B-NH2.
- Tk and acquired B antigens are distinct entities, despite some serological similarities.