Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Malachite green-INT (MINT) method for determining active bacteria in sewage.

R J Dutton, G Bitton, B Koopman

    Applied and Environmental Microbiology
    |December 1, 1983
    PubMed
    Summary

    A new membrane filtration method accurately quantifies active bacteria in sewage treatment. This technique simplifies measuring respiring bacteria, crucial for understanding wastewater processes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Molecular Tumor Board of the University Medical Center Groningen (UMCG-MTB): outcome of patients with rare or complex mutational profiles receiving MTB-advised targeted therapy.

    ESMO open·2024
    Same author

    Recovery of Coliphages from Chicken, Pork Sausage and Delicatessen Meats.

    Journal of food protection·2019
    Same author

    A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells.

    Lab on a chip·2014
    Same author

    Standardized modulation of the injection site allows for insulin dose reduction without deterioration of metabolic control.

    Current medical research and opinion·2014
    Same author

    Speed-dependent reference joint trajectory generation for robotic gait support.

    Journal of biomechanics·2014
    Same author

    The Barmer study: impact of standardized warming of the injection site to enhance insulin absorption and reduce prandial insulin requirements and hypoglycemia in obese patients with diabetes mellitus.

    Current medical research and opinion·2014

    Area of Science:

    • Environmental microbiology
    • Wastewater treatment technologies

    Background:

    • Assessing bacterial activity is vital for optimizing sewage treatment processes.
    • Existing methods for determining active bacteria are often time-consuming and complex.

    Purpose of the Study:

    • To develop a rapid and simplified membrane filtration method for quantifying active (respiring) bacteria in sewage.
    • To evaluate the proportion of active bacteria at different stages of sewage treatment.

    Main Methods:

    • Incubation of samples with 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT).
    • Fixation and filtration of samples through membrane filters.
    • Counterstaining with malachite green and examination via bright-field microscopy after filter clearing for enhanced contrast.

    Main Results:

    • Active bacterial fractions were determined as 40% in raw sewage, 29% in settled sewage, and 58% in secondary effluent.
    • The proportion of respiring bacteria in chlorinated secondary effluent was 6.1%.
    • The active bacterial fraction in activated sludge was found to be 16%.

    Conclusions:

    • The developed membrane filtration method offers a significant improvement in speed and simplicity for determining active bacteria in sewage.
    • This method provides valuable data for monitoring and managing wastewater treatment efficiency.

    Related Experiment Videos