Antibody against globular domain of H10 histone

Antibodies against the globular domain of histones H10 and H5 were developed in rabbit. The antibody against the globular domain of H5 cross-reacted with H10 but not with H1; the antibody against the globular domain of H10 did not cross-react with H5, H1 or with HMG proteins. The globular domain of H10 therefore appears to have an immunological determinant(s) which does not exist in H1 and H5. By use of these antibodies, we show that nucleated erythrocytes of bullfrog contain an H10-like protein (not an H5-like protein). This observation coincided with the report of Shimada, T. et al. [J. Biol. Chem. 256 (1981) 10577-10582]. These antibodies have application in detecting H10-like proteins in eukaryotic cells.