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[A method for the cryopreservation of thrombocytes]
Folia Haematologica (Leipzig, Germany : 1928)
|January 1, 1983
Summary
Cryopreservation of thrombocyte concentrates using dimethylsulfoxide (DMSO) maintains 50% in vitro function and comparable in vivo survival to fresh platelets. This enables the creation of HLA-typed single-donor pools for clinical use.
Area of Science:
- Hematology
- Cryobiology
- Transfusion Medicine
Context:
- Thrombocyte concentrates are crucial for treating bleeding disorders.
- Cryopreservation offers a method for long-term storage of blood components.
- Developing effective cryopreservation techniques for platelets is essential for transfusion services.
Purpose:
- To develop a cryopreservation technique for thrombocyte concentrates using dimethylsulfoxide (DMSO).
- To evaluate the in vitro functional ability and in vivo survival of cryopreserved thrombocytes.
- To establish the feasibility of creating HLA-typed single-donor pools for clinical application.
Summary:
- A cryopreservation technique for thrombocyte concentrates was developed using 0.7 mol/l dimethylsulfoxide (DMSO).
- In vitro testing showed a mean functional ability of 50% compared to fresh thrombocytes.
- In vivo survival studies indicated that cryopreserved thrombocytes have a survival time comparable to fresh cells.
Impact:
- The study demonstrates the potential for long-term storage of functional thrombocytes.
- This technique facilitates the establishment of HLA-typed single-donor platelet pools.
- Cryopreserved thrombocytes can improve the availability and clinical application of platelet transfusions.