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Endogenous avidin-binding activity in human lymphoid tissue.

D Banerjee, S Pettit

    Journal of Clinical Pathology
    |February 1, 1984
    PubMed
    Summary
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    Endogenous avidin binding activity (EABA) in human lymphoid tissues can cause false positive staining in biotin-avidin detection systems. Blocking EABA with avidin and biotin prior to staining ensures accurate T lymphocyte subset identification in cryostat sections.

    Area of Science:

    • Immunohistochemistry
    • Cell Biology
    • Histology

    Background:

    • Biotin-avidin systems offer an alternative to indirect antibody sandwich methods for detecting T lymphocyte subsets in human lymphoid tissue cryostat sections.
    • Endogenous avidin binding activity (EABA) is present in human lymph nodes and tonsils, potentially leading to false positive staining results.

    Purpose of the Study:

    • To investigate the source and implications of endogenous avidin binding activity (EABA) in human lymphoid tissues.
    • To develop a method to mitigate false positive staining caused by EABA in biotin-avidin detection systems.

    Main Methods:

    • Analysis of cryostat sections from human lymph nodes and tonsils.
    • Assessment of endogenous avidin binding activity (EABA) in various cell populations.

    Related Experiment Videos

  • Morphological and peroxidase activity evaluation of avidin-binding cells.
  • Application of an avidin-biotin blocking procedure before specific staining.

    • Main Results:

    • Significant EABA was detected in human lymph nodes and tonsils, causing false positive staining.
    • Avidin-binding cells exhibited endogenous peroxidase activity and morphological similarities to histiocytes.
    • EABA was absent in intrafollicular macrophages and rare in medullary sinus histiocytes, suggesting a specific cell lineage.
    • Treatment with avidin followed by biotin effectively blocked EABA.

    Conclusions:

    • EABA in lymphoid tissues can interfere with accurate T lymphocyte subset detection using biotin-avidin methods.
    • The lineage of avidin-binding cells requires further investigation.
    • A blocking strategy using avidin and biotin effectively prevents EABA, enabling reliable detection of target cells.