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Related Experiment Videos

Partial characterization of physiologically generated C3 components expressing C3d but not C3c epitopes.

H C Siersted, J C Jensenius, S E Svehag

    Journal of Clinical & Laboratory Immunology
    |December 1, 1983
    PubMed
    Summary

    This study quantifies four C3d plasma protein components, identifying a 45K C3d fragment (d2) as a likely final product of complement C3 breakdown in human serum.

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    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • Complement system activation involves the breakdown of C3.
    • C3d is a key fragment indicating complement activation.

    Purpose of the Study:

    • To quantify plasma protein components containing C3d epitopes.
    • To characterize these C3d-containing components and their role in C3 breakdown.

    Main Methods:

    • Quantification of C3d using specific techniques.
    • Radiolabelling of C3 and plasma supernatants.
    • Analysis using SDS-PAGE, autoradiography, and crossed immunoelectrophoresis.

    Main Results:

    • Identified and characterized four C3d-containing plasma protein components (d1, d1', d2, d3).
    • Determined molecular weights and electrophoretic mobilities of these components.

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  • Proposed the 45K d2 component as a final physiological breakdown product of C3.

    • Conclusions:

    • The identified C3d components are derived from C3.
    • These components are valuable indicators of complement activation in clinical settings.
    • The 45K d2 fragment represents a significant finding in understanding C3 catabolism.