Interferon activity produced by interferon messenger RNA in Tilapiamosaiabica peters oocytes

The activities of interferon mRNA from various types of mouse cells have been studied using Tilapiamosaiabica peters oocytes (TPO) as the translation system. The translation activity of interferon mRNA in TPO was also compared with that in Xenopus laevis oocytes (XLO). The results obtained showed similar translation efficiency in both systems. The highest translation efficiency was observed when T. peters oocytes, microinjected with mRNA, were cultivated in Eagle's medium containing 0.01% HEPES and 0.5% NaHCO3 (pH 7.5) at 25 degrees C. pppA2' P5' A2' P5' A in concentration of 500 nM did not affect the translation of interferon mRNA in oocytes, but inhibition was demonstrated at 1 microM. A linear relationship was found between the amount of interferon mRNA injected and the antiviral activity of the translation products, suggesting that the amount of interferon mRNA can be determined by assaying the interferon activity of its translation product.