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Fine-specificity of the lambda and chi L chains associated with antibodies directed to alpha (1 leads to 3) glucosyls
The function of BALB/c primary B precursors, responding to dextran (dex) B-1355, and the fine-specificity of the B-1355-binding, lambda and chi, monofocal antibody (Ab) generated by the precursors have been examined. In splenic fragments from Limulus polyphemus haemocyanin (LPH)-primed, lethally irradiated, euthymic or nu/nu BALB/c mice cultured with thymus-independent (TI) dex B-1355, B-1355-lipopolysaccharide (LPS), B-1355-LPS-LPH, or thymus-dependent (TD) dex B-1355-LPH, the lambda 1 precursors responded with B-1355-binding Ab substantially equally with respect to precursor frequency, rate of Ab production, and range of fine-specificity, but not with respect to frequency of the IdX and IdI isotypes related to the VH and DH associated with the lambda 1. The lambda 2 contributed minimally to the repertoire. The chi precursors responded with B-1355-binding Ab at a rate nearly equal to the lambda 1 only under TD stimulus in euthymic fragments. A comparison of the lambda 1 and chi Ab fine-specificity, by inhibition of binding with dex differing in epitope contents and configurations, showed marked restriction in the chi relative to the lambda 1. Only approximately 10% of the relatively more abundant chi TD response showed fine alpha (1 leads to 3) specificity similar to that of the lambda 1. The lambda 1 fine-specificity diversity resided mainly in the IdI fraction.
The function of BALB/c primary B precursors, responding to dextran (dex) B-1355, and the fine-specificity of the B-1355-binding, lambda and chi, monofocal antibody (Ab) generated by the precursors have been examined. In splenic fragments from Limulus polyphemus haemocyanin (LPH)-primed, lethally irradiated, euthymic or nu/nu BALB/c mice cultured with thymus-independent (TI) dex B-1355, B-1355-lipopolysaccharide (LPS), B-1355-LPS-LPH, or thymus-dependent (TD) dex B-1355-LPH, the lambda 1 precursors responded with B-1355-binding Ab substantially equally with respect to precursor frequency, rate of Ab production, and range of fine-specificity, but not with respect to frequency of the IdX and IdI isotypes related to the VH and DH associated with the lambda 1. The lambda 2 contributed minimally to the repertoire. The chi precursors responded with B-1355-binding Ab at a rate nearly equal to the lambda 1 only under TD stimulus in euthymic fragments. A comparison of the lambda 1 and chi Ab fine-specificity, by inhibition of binding with dex differing in epitope contents and configurations, showed marked restriction in the chi relative to the lambda 1. Only approximately 10% of the relatively more abundant chi TD response showed fine alpha (1 leads to 3) specificity similar to that of the lambda 1. The lambda 1 fine-specificity diversity resided mainly in the IdI fraction.