[Visualization of different types of hapten-cell membrane interaction by fluorine high-field nuclear magnetic...

Using a high field spectrometer (5.9 Tesla) 19F-NMR spectrum of soluble material from 4 trifluoromethyl 2,6 dinitrobenzene sulphonate (CF3-DNBS) treated murine lymphocytes was recorded. CF3-DNBS is a fluorinated analog of 2,4,6 trinitrobenzene sulphonate (TNBS), and these compounds have been found to create cross-reacting antigenic modifications of cell surface. At least 4 distinguishable signals have been detected, and we think that 19F-NMR could be used to study, at a molecular level, some immunochemical problems concerning modification with TNBS.