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Changes in cell surface antigens during stem cell ontogeny.

M V Berridge, S J Ralph, A S Tan

    Experimental Hematology
    |February 1, 1984
    PubMed
    Summary
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    Murine pluripotent stem cell (CFU-s) surface antigens change dramatically during development, with distinct antibody groups revealing developmental and strain-specific profiles. These findings illuminate stem cell heterogeneity and ontogeny.

    Area of Science:

    • Developmental immunology
    • Stem cell biology
    • Hematopoiesis

    Background:

    • Murine pluripotent stem cells, specifically colony-forming unit-spleen (CFU-s), are crucial for hematopoiesis.
    • Understanding the cell surface antigens of CFU-s during development is key to characterizing stem cell populations.
    • Monoclonal antibodies provide precise tools for dissecting cell surface antigen expression.

    Purpose of the Study:

    • To investigate the changing antigenic profile of murine pluripotent stem cells (CFU-s) throughout ontogeny.
    • To identify distinct antigen groups on CFU-s and their developmental regulation.
    • To explore strain-specific variations in CFU-s antigen expression.

    Main Methods:

    • Utilized a panel of monoclonal antibodies targeting murine CFU-s.

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  • Examined antigen expression across different developmental stages (fetal liver to adult bone marrow and spleen).
  • Employed quantitative absorption analysis and complementation studies to differentiate antibody specificities.

    • Main Results:

    • Two distinct groups of CFU-s antigens were identified based on their developmental expression patterns.
    • One antigen group showed plateau expression, increasing marginally with development, and was strain-invariant.
    • A second antigen group, including anti-H-2Kk, increased significantly from fetal liver to adult bone marrow and exhibited strain-specific variations.

    Conclusions:

    • Murine stem cell surfaces undergo significant antigenic changes during ontogeny.
    • Distinct antigen expression patterns reflect heterogeneity within the CFU-s population.
    • These findings contribute to understanding stem cell development and potential immunomodulation targets.