Neoplastic transformation by E26 leukemia virus is mediated by a single protein containing domains of gag and myb genes
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Journal of Virology
|April 1, 1984
Abstract:
The genome of avian leukemia virus E26 shares homology with v-myb, the oncogene of avian myeloblastosis virus, and encodes a protein with an Mr of 135,000. Analyses of tryptic oligopeptides show that this protein is related to the proteins encoded by gag (Pr76gag) as well as v-myb (p45v-myb[AMV] ) and c-myb (p75c-myb). We found no evidence for the existence of additional myb-related proteins or subgenomic species of myb-related RNA in myeloblasts transformed by strain E26.
Insights
Avian leukemia virus E26 encodes a novel 135,000 Mr protein. This protein is related to gag, v-myb, and c-myb oncogenes, with no evidence of additional myb-related proteins.
Area of Science:
- Oncology
- Virology
- Molecular Biology
Background:
- Avian leukemia virus E26 (E26) is an oncogenic retrovirus.
- The v-myb oncogene from avian myeloblastosis virus (AMV) is crucial for myeloid leukemogenesis.
Purpose of the Study:
- To characterize the protein encoded by the E26 viral genome.
- To investigate the relationship of the E26-encoded protein to known myb proteins.
Main Methods:
- Genome analysis of avian leukemia virus E26.
- Tryptic oligopeptide analysis of the encoded protein.
- Comparison with gag, v-myb, and c-myb proteins.
Main Results:
- The E26 genome encodes a 135,000 Mr protein.
- This protein shows homology to gag (Pr76gag), v-myb (p45v-myb[AMV]), and c-myb (p75c-myb).
- No additional myb-related proteins or RNA species were detected in E26-transformed myeloblasts.
Conclusions:
- The E26 virus encodes a unique myb-related protein.
- This protein's relationship to gag and myb oncogenes provides insights into viral oncogenesis.
- Further studies are warranted to elucidate the specific role of this protein in E26-induced leukemia.