Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Identifier sequences are transcribed specifically in brain.

J G Sutcliffe, R J Milner, J M Gottesfeld

    Nature
    |March 15, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Long intronic GAA*TTC repeats induce epigenetic changes and reporter gene silencing in a molecular model of Friedreich ataxia.

    Nucleic acids research·2008
    Same author

    Radioligand binding analysis of knockout mice reveals 5-hydroxytryptamine(7) receptor distribution and uncovers 8-hydroxy-2-(di-n-propylamino)tetralin interaction with alpha(2) adrenergic receptors.

    Neuroscience·2004
    Same author

    Histone-DNA binding free energy cannot be measured in dilution-driven dissociation experiments.

    Biochemistry·2004
    Same author

    Antipsychotic drug treatment alters expression of mRNAs encoding lipid metabolism-related proteins.

    Molecular psychiatry·2003
    Same author

    From pharmacotherapy to pathophysiology: emerging mechanisms of apolipoprotein D in psychiatric disorders.

    Current molecular medicine·2003
    Same author

    Differences in neuroanatomical sites of apoD elevation discriminate between schizophrenia and bipolar disorder.

    Molecular psychiatry·2003
    Same journal

    Daily briefing: How cooperation built the world.

    Nature·2026
    Same journal

    Deep-sea oddities and boatloads of other new species - June's best science images.

    Nature·2026
    Same journal

    From cloning to gene-editing: the enduring legacy of Dolly the sheep.

    Nature·2026
    Same journal

    Time to give hydration breaks the red card? What science says about keeping cool.

    Nature·2026
    Same journal

    Universities are relying on AI-detection software to catch cheating. How well do the programs work?

    Nature·2026
    Same journal

    Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

    Nature·2026
    See all related articles

    Brain cells utilize specific RNA sequences (ID sequences) for gene activation via RNA polymerase III transcription. This process is unique to neural tissues, unlike other cell types.

    Area of Science:

    • Molecular Biology
    • Neuroscience
    • Genetics

    Background:

    • RNA polymerase II and III are crucial for gene transcription.
    • Specific RNA sequences, termed 'Identifier' or ID sequences, are found in transcripts.
    • Neural tissues exhibit unique molecular characteristics compared to other tissues.

    Purpose of the Study:

    • To investigate the role and prevalence of ID sequences in different tissue types.
    • To understand the function of BC1 and BC2 RNA species in vivo.
    • To elucidate the mechanism of brain-specific gene activation involving ID sequences.

    Main Methods:

    • In vitro transcription assays using brain and non-brain nuclei.
    • Analysis of cytoplasmic poly(A)+ RNA species (BC1 and BC2) in vivo.

    Related Experiment Videos

  • Cloning of ID sequences and assessment of their template activity for polymerase III.

    • Main Results:

    • ID sequences were found in 62% of RNA polymerase II and III transcripts from brain nuclei, but less than 4% in other tissues.
    • Homologous 160-nucleotide BC1 and smaller BC2 RNA species were exclusively located in neural tissues in vivo.
    • Cloned ID sequences function as templates for RNA polymerase III in vitro.

    Conclusions:

    • A model is proposed where brain-specific RNA polymerase III transcription of intronic ID sequences activates target genes.
    • This mechanism represents a primary mode of gene activation in the brain.
    • The findings highlight a unique transcriptional regulatory pathway in neural development and function.