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A cell membrane "gp70" associated with Fv-4 gene: immunological characterization, and tissue and strain distribution
Abstract:
Fv-4r is a dominant resistance gene which controls susceptibility of mice to exogenous infection with ecotropic murine leukemia virus (MuLV). A MuLV env-gene product related to gp70 on cells from uninfected Fv-4r mice has been found. Expression of this molecule is closely linked to the resistance allele. Antigenic determinants on the Fv-4r gp70 are similar to many ecotropic and some dualtropic MuLV gp70s but different from xenotropic and amphotropic MuLV gp70s. The Fv-4r gp70 molecule also has unique antigenic determinants since it is recognized by Fv-4 specific antisera which do not recognize other cell membrane gp70s or viral gp70s. The Fv-4-specific cell membrane gp70 is detectable on cells from thymus, spleen, lymph node, bone marrow, and embryo fibroblasts from Fv-4r mice, but not from Fv-4s mice. Slight differences in electrophoretic mobility of Fv-4r gp70 are found on different tissues. This unique cell membrane gp70 may be causally related to Fv-4 resistance.
Insights
Mice with the Fv-4r gene resist ecotropic murine leukemia virus (MuLV) infection. A unique gp70 molecule on Fv-4r mouse cells is linked to this resistance, suggesting a novel antiviral mechanism.
Area of Science:
- Virology
- Immunogenetics
Background:
- The Fv-4r gene confers dominant resistance in mice against ecotropic murine leukemia virus (MuLV) infection.
- Understanding the molecular basis of Fv-4r-mediated resistance is crucial for developing antiviral strategies.
Purpose of the Study:
- To investigate the molecular nature of the Fv-4r resistance gene.
- To identify the specific viral or cellular products associated with Fv-4r resistance.
Main Methods:
- Analysis of cell surface gp70 expression in Fv-4r resistant mice.
- Immunological characterization of Fv-4r-associated gp70 using specific antisera.
- Detection of Fv-4r gp70 across various tissues and comparison of electrophoretic mobility.
Main Results:
- A novel gp70 molecule, related to the MuLV env-gene product, was identified on cells from Fv-4r mice.
- This Fv-4r gp70 exhibits unique antigenic determinants, distinct from other MuLV gp70s, and is recognized by Fv-4 specific antisera.
- Fv-4r gp70 expression was detected in thymus, spleen, lymph nodes, bone marrow, and embryo fibroblasts, with tissue-specific electrophoretic mobility differences.
Conclusions:
- The Fv-4r resistance allele is associated with the expression of a unique cell membrane gp70 molecule.
- This Fv-4r-specific gp70 is a strong candidate for mediating resistance to ecotropic MuLV infection in mice.