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Updated: Aug 13, 2026

Isolation, Fixation, and Immunofluorescence Imaging of Mouse Adrenal Glands
Published on: October 2, 2018
Cell free translation of rat adrenal messenger RNA coding for an ACTH-induced protein
Abstract:
Protein markers induced by hormones are the necessary probes to study hormone regulation of gene expression. We have previously shown that ACTH was able to induce one of these markers in the cytosol of the rat adrenal: protein E (Dazord et al, Biochem. J., 176, 233-239, 1978). In this paper by using adrenal mRNA from control and in vivo ACTH treated rats as well as protein E antibodies, we show that: 1) ACTH affects both the amount of adrenal mRNA and its translational activity; 2) the translation of protein E is increased by the hormone; 3) protein E is the major translational product following ACTH treatment.
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